Privacy Policy. We will not share your information for any other purposes. ... protein encoded by this gene is erythrocyte-specific and is thought to be part of a membrane channel that transports ammonium and carbon dioxide across the blood cell membrane. are provided for Customer as the end-user and solely for research and development uses. The treated proteins are then dissolved in ammonium bicarbonate buffer (0.05 mol l − 1, pH 8.4) to a concentration of 2 mg ml − 1 and TPCK-treated trypsin (l-(1-tosylamide-2-phenylethyl chloromethyl ketone)) is added to give a final enzyme-to-substrate ratio of 1:75. The pK a values of formic acid and the ammonium ion are 3.8 and 9.2, respectively. * Definitions are provided on page 5. Ammonium hydrogen carbonate is an excellent buffer for use at high-pH with good buffering capacity over pH 8-11 and possibly wider at higher ionic strength. 2 mM Ammonium Acetate buffer, pH-adjusted to 4.0. Stability in 10 mM ammonium bicarbonate, pH 10 A second experiment was performed using 10 mM ammonium bicarbonate at pH 10 (Figure 2). 1M Ammonium bicarbonate buffer in HPLC water is provided for use with Cell Signaling Technology's patented PTMScan® protocol. EC Number 213-911-5. HPLC Column Choices for the Analysis of Proteins and Peptides . CST's Product Terms of Sale and any applicable or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. It is a colourless solid that degrades readily to carbon dioxide, water and ammonia. When heated, ammonium formate eliminates water, forming formamide. Any Customer's terms and conditions that are in Ammonium bicarbonate buffer is supplied as a one molar (1M) concentrated stock in HPLC grade water. Would you like to visit your country specific website? Beilstein/REAXYS Number 4329606 . Poly Bottle; 500g. MDL number MFCD00012138. Ammonium acetate CH 3CO 2NH 4 4.8 3.8–5.8 4-Methylmorpholine OC 4H 8N(CH 3) 8.4 7.4–9.4 Ammonium bicarbonate NH 4CO 3H 6.3/9.2/10.3 6.8–11.3 Ammonium acetate CH 3CO 2NH 4 9.2 8.2–10.2 Ammonium formate HCO 2NH ... phase from the HPLC into the interface and run a scan to see if the back-ground decreases. This mobile phase helps to control pH as it has good buffering capacity, and it allows commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying requires a separate license from CST. For more information or to place an order contact: The Nest Group, Inc.™ 10 Southville Rd, Suite 4, Southborough, MA 01772-4026 USA Tel: 1-508-481-6223 Fax: 1-508-485-5736 For your convenience, we accept Mastercard, VISA, and American Express credit cards. Shop a large selection of Ammonium products and learn more about Ammonium Carbonate (Lumps/HPLC), Fisher Chemical. Ammonium bicarbonate does this in the pH 8 to 10 range. Molecular Weight 79.06 . Ammonium buffers in the alkaline range, but it is a component of the ammoniumbicarbonate anyway. Edit this message by logging in to your account, Note: Product prices and availability are subject to change without notice, Electrophoresis, Western Blotting and ELISA, Chromatography and Mass Spectrometry Reagents, Chlorine and pH Test Kits and Test Strips, Applied Biosystems™ TaqMan® Assay and Arrays Search Tool, Applied Biosystems™ TaqMan® Custom Assay Design Tools, Applied Biosystems™ Custom qPCR Primers and TaqMan® Probes Tool, Suitable for preparative chromatography and HPLC, Unique blend formulated to improve separation of compounds relative to conventional acid-modified mobile phases, Developed in collaboration with the Thermo Fisher Scientific chromatography experts. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PTMScan is a trademark of Cell Signaling Technology, Inc. Water, LC-MS Grade (Burdick and Jackson™). This buffer is recommended for use in PTMScan protocols for … Your feedback has been submitted. Page 2 ... ammonium sulfate, lithium sulfate, sodium perchlorate) gradient. If a low-pH mobile phase is all that is important, 0.1% v/v phosphoric acid Several buffers are sufficiently volatile to qualify for LC-MS use. Ammonium bicarbonate (also known as ammonium hydrogen carbonate) is a mildly basic inorganic compound with the formula (NH4)HCO3. C = O N-C C = H-N C H 2 H N-C O = C-H H-CH 2 C CH 2 CH 2 NH 2 H O H O H Hydrophobic Interaction Chromatography (HIC) Page 7 Separation of Protein Mixture Using Just Interested in Low pH? It is a colourless, crystalline solid with the odour of ammonia. Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms This buffer is recommended for use in PTMScan protocols for LC-MS applications during the digestion procedures. Contrary to com-mon expectations, high pH mobile phases do NOT suppress the ionization of basic compounds in ESI+; positive ions are formed abundantly, and analyte responses are often better in high pH compared to acidic mobile phases. Special Populations Precaution. Sodium bicarbonate softens water, neutralizes acids, absorbs odors, and provides a mild abrasive for cleaning. Ammonium bicarbonate is an inorganic compound with formula (NH 4 )HCO 3, simplified to NH 5 CO 3. It is used in baking powders and fire extinguishers, and to make dyes and pigments. Ammonium Carbonate, for HPLC, Certified, Fisher Chemical Brand: Fisher Chemical A/3686/50 Code : 25. Pronounced: ah-MOH-nee-uhm by-KAR-boh-nayt. Better consult to your doctor if you would like to intake Ammonium Bicarbonate. services used by Customer in connection with the Products. A leavening agent, and the forerunner of today's baking powder and baking soda. Fisher Scientific is always working to improve our content for you. It is soluble in water, but insoluble in most organic solvents including ethanol, carbon disulphide and concentrated ammonia. The compound has many names, reflecting its long history. Ammonium Bicarbonate can be used in Food, Beverage, Pharmaceutical, Health & Personal care products, Agriculture/Animal Feed/Poultry. Thermo Scientific™ pH 10 HPLC Mobile Phase Buffer, 10mM Ammonium Bicarbonate, 5% Methanol High pH (10.0) mobile phase improves chromatographic separation of compounds relative to conventional acid-modified mobile phases in HPLC. Secondary trypsin digestion of enriched LysC or Methylated peptides is recommended for all absophillic and methylation-specific motif antibodies. Originally made from the ground antlers of reindeer, this is an ancestor of modern baking powder. BJ40867-50GEA 57.3 USD. Acetate and formate buffer in the acidic range, not in the alkaline range. The solution should preferably be … T006024000 Chemically speaking, it is the bicarbonate salt of the ammonium ion. We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. human (1) Biological Source. Ammonium Bicarbonate vs Sodium Bicarbonate – Differences. Ammonium bicarbonate BioUltra, ≥99.5% (T) Synonym: Ammonium hydrogen carbonate CAS Number 1066-33-7. representative of CST, are rejected and are of no force or effect. Manufacturer:  Thermo Scientific™ T006024000. Nutrition. Ammonium hydrogen carbonate CAS 1066-33-7 for HPLC LiChropur® - Find MSDS or SDS, a COA, data sheets and more information. Any use of Product for diagnostic, compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or All contact information provided shall also be maintained in accordance with our apply to Products provided by CST, its affiliates or its distributors. by the FDA or other regulatory foreign or domestic entity, for any purpose. Please sign in to view account pricing and product availability. Reactions. Due to product restrictions, please Sign In to purchase or view availability for this product. structure or technology of the Products, or use the Products for the purpose of developing any products or services that would Use this 1M ammonium bicarbonate (20X) stock to prepare the final digestion buffer of 50mM ammonium bicarbonate with 5% acetonitrile. High‐pH or basic/alkaline mobile phases are not commonly used in LC–MS or LC–MS/MS bioanalysis because of the deeply rooted concern with column instability and reduced detection sensitivity for basic compounds in high‐pH mobile phases owing to charge neutralization. The extended buffering range is due to the ammonia – ammonium buffering capacity being additive to the hydrogen carbonate-carbonate capacity in what is traditionally called a ‘mixed buffer’. Ammonium Bicarbonate is considered safe by FDA. Let’s say that according to the pKa of your analytes, you need to prepare a solution with a pH of 4.0. Ammonium Bicarbonate is a white powder or crystalline (sand-like) material with an Ammonia odor. copyright notices or markings, (d) use the Products solely in accordance with BJ40867-50G. documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or HPLC (1) microbiological culture (2) Host Species. Ammonium bicarbonate (6.6